潘孝明

 

潘孝明，男，工学博士，讲师，2014年6月毕业于中国海洋大学。

现授课程：《食品生物技术》、《转基因食品安全分析》

目前研究方向或领域：

微生物检测，DNA扩增检测

目前承担的科研项目：

[1] 牡蛎中四种主要致病菌的同步高灵敏度检测方法研究，山东省高等学校科技计划项目，2万元，J18KA140，主持，在研。

[2] 预扩增PCR-LAMP法对水产品中致病菌检测研究，农业部水产品加工重点实验室开放基金课题，NYJG201801，2万元，主持，在研。

[3] 新型预扩增法及其在对虾白斑症病毒检测鉴定中的应用，中国博士后科学基金面上项目，2015M572086，5万元，主持，已结题。

[4] 复杂背景中极微量目标DNA的高灵敏度检测及应用，青岛市博士后研究人员应用研究项目，5万元，主持，已结题。

目前有代表性的论文：

1.    Pan, X., Wan, B., Li, C., Liu, Y., Wang, J., Mou, H., Liang, X. A novel whole genome amplification method using type IIS restriction enzymes to create overhangs with random sequences. Journal of Biotechnology, 2014, 184: 1-6.

2.    Pan, X., Wang, J., Zhang, Y., Dong, P., Li, C., Liang, X. Detection of trace amounts of target DNA from massive background of nucleic acids by using the LM‐PCR‐based preamplification method. Biotechnology and applied biochemistry, 64(6), 879-887.

3.    Pan, X., Zhang, Y., Sha, X., Wang, J., Li, J., Dong, P., Liang, X. Highly sensitive detection of low-abundance of white spot syndrome virus by a pre-amplification PCR method. Journal of Microbiology and Biotechnology. 2017, 27(3): 471~479.

4.    Pan, X., Chen, W., Jia, X., Dong, P., Liang, X. Evaluation of a Whole Genome Amplification Method Based on Improved Ligation-Mediated PCR. Lecture Notes in Electrical Engineering, 2014, 251: 1333-1343.

5.    Wang, J., Pan, X., Liang, X. Assessment for Melting Temperature Measurement of Nucleic Acid by HRM. Journal of Analytical Methods in Chemistry, 2016, 2016: 5318935.

6.    Zhang, Y., Wang, X., Pan, X., Liu, Y., Wang, H., Dong, P., Liang, X. The effects of food components on the digestion of DNA by pepsin. International Journal of Food Sciences and Nutrition, 2016, 67(7): 797-805.

7.    Wang, J., Dong, P., Wu, W., Pan, X., Liang, X. High-throughput thermal stability assessment of DNA hairpins based on high resolution melting. Journal of Biomolecular Structure & Dynamics, doi: 10.1080/07391102.2016.1266967.